相关实验视频

Updated: Jun 28, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

在缓慢生长,非分泌,黑色化,内菌的有效基因编辑 Berkleasmium sp. Dzf12使用一个CRISPR/Cas9系统

Siji Zhao¹, Ruya Yin¹, Mengwei Zhang¹

¹Department of Plant Pathology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian district, Beijing, 100193, China.

World journal of microbiology & biotechnology

|April 23, 2024

概括

研究人员开发了一个CRISPR/Cas9基因编辑系统用于真菌Berkleasmium sp. Dzf12,能够有效地破坏基因,并有助于研究棕胺生产.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
生物技术是生物技术.

背景情况:

伯克莱亚斯 sp. 的一种. 迪奥斯科雷亚 Zingiberensis 的一个内菌 Dzf12,产生生物活性的palmarumycins.
这种真菌的遗传操纵是具有挑战性的,因为它的生长速度缓慢,缺乏子和色素.

研究的目的:

开发和优化CRISPR/Cas9系统,以便在Berkleasmium sp.中有效编辑基因. 在 Dzf12.12 中.
通过准参与黑色素生物合成的基因来验证系统的有效性.

主要方法:

优化了原生质细胞的制备和验证了Cas9的表达.
针对破坏的假定的1,3,6,8-四氨酸甲合成酶基因 (bdpks).
对比内源性sgRNA促进体,确定U6snRNA-1促进体是最优的.

主要成果:

在Berkleasmium sp.中成功破坏了bdpks基因. 在 Dzf12.12 中.
基因干扰导致了螺旋双纳和黑色素的生产完全停止.
为这种难以操纵的真菌建立了一个高效的CRISPR/Cas9系统.

结论:

开发的CRISPR/Cas9系统有助于在Berkleasmium sp.中进行功能性基因探索. 在 Dzf12.12 中.
这项工作为将CRISPR/Cas9应用于其他具有挑战性的真菌物种提供了一个模型.

关键词:

伯克利斯米亚姆是一个.这是一个CRISPR/Cas9系统.这是内性真菌.基因编辑基因编辑黑色素是黑色素的一种.斯皮罗比斯纳夫他林是一种.

更多相关视频

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...