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生物标志物的组合,用于预测马的精子生育能力
Anders Johannisson1,2, Jane M Morrell3, Theodoros Ntallaris1
1Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
Veterinary research communications
|April 23, 2024
概括
需要对马匹提供可靠的生育生物标志物. 精子运动性,膜完整性,DNA碎片化和活性氧物种显示出预测人工授精后马匹生育能力的前景.
科学领域:
- 兽医生殖的动物生殖方式
- 动物科学动物科学
- 生殖生物学 生殖生物学
背景情况:
- 准确预测马生育率对于有效的马匹育种计划至关重要.
- 目前评估射精质量的方法在预测体内生育结果方面存在局限性.
研究的目的:
- 评估各种体外精子特征与人工授精后马匹的生育率之间的相关性.
- 探索开发基于客观精子参数的马生育预测模型的潜力.
主要方法:
- 来自多只马的精液样本在采集后24-30小时内被分析.
- 实验室精子参数的评估包括膜完整性,体完整性,染色质完整性,线粒体膜潜力和通过流动细胞计量产生反应性氧物种.
- 用计算机辅助的精子分析来评估精子运动性.
- 每个季节的怀孕率在第二年被记录下来用于相关性分析.
主要成果:
- 在怀孕率和精子直度 (r=0.43) 和活的过氧化阳性精子 (r=0.32) 之间观察到正相关性.
- 在怀孕率和横向头部位移幅度 (r=-0.26) 和死亡超氧化物阳性精子 (r=-0.46) 之间发现了负相关性.
- 主要成分分析表明,运动性,膜完整性,DNA碎片化和反应性氧物种的产生与怀孕率有关.
结论:
- 精子运动性,膜完整性,DNA碎片化和反应性氧物种生产的组合可以作为马生育能力的潜在生物标志物.
- 需要对更大的数据集进行进一步的研究,以开发一个强大的子生育预测模型.
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