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MAB-5/Hox调节Q神经细胞转录组,包括cwn-1/Wnt,以调节Caenorhabditis elegans的后部迁移

Vitoria K Paolillo¹, Matthew E Ochs¹, Erik A Lundquist¹

¹Department of Molecular Biosciences, KU Center for Genomics, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA.

|April 23, 2024

概括

转录因子MAB-5通过调节cwn-1等基因来重新编程Q神经母细胞以进行后部迁移. 这项研究确定了MAB-5受控制的基因,这些基因对C. elegans的神经发生和神经元发育至关重要.

科学领域:

发展生物学发展生物学
神经科学是一个神经科学.
遗传学是一个遗传学.

背景情况:

神经生成需要精确的遗传控制,以进行神经元的分化和迁移.
在C. elegans中,Q神经细胞 (QL和QR) 呈现出类似的分裂模式,但不同的迁移方向 (QL的后部,QR的前部).
MAB-5/Hox转录因子是后部迁移的关键,通常表达在QL血统中.

研究的目的:

在早期神经发生过程中识别Q细胞中由MAB-5调节的基因.
阐明MAB-5介导的Q系迁移背后的分子机制.
定义CWN-1在MAB-5功能中的作用.

主要方法:

光激活细胞分类 (FACS) 用于从C. elegans幼虫中分离Q细胞.
从野生类型和突变菌株 (mab-5功能损失和功能增益) 中分类Q细胞的RNA测序 (RNA-seq) 和差异基因表达分析.
功能遗传分析,以评估基因在QL迁移中的作用.

主要成果:

鉴定了参与细胞分裂,DNA复制和Q细胞修复的基因,反映了它们的神经细胞的身份.
发现的MAB-5调节与神经发生,神经发育和细胞外矩阵相互作用相关的基因.
发现cwn-1表达被MAB-5直接控制,在mab-5功能丧失中表达减少,在mab-5功能获取突变体中表达增加.
证明CWN-1作用于MAB-5的下游,以抑制前部QL迁移,可能通过非正规的Wnt途径.

结论:

MAB-5对于重新编程Q系细胞以进行后部迁移和防止前部迁移至关重要.
该研究提供了Q细胞的转录组和一组MAB-5调节基因.
CWN-1是MAB-5的下游效应因子,在调节QL细胞迁移方向方面发挥着关键作用.

关键词:

在C elegans中使用.在CWN-1/Wnt.在MAB-5/Hox中使用.在RNA-seqqq.用光激活的细胞分类进行分类.神经细胞迁移的迁移

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