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perfluorinated 化学品致癌潜力的体外评估

Monica Vaccari¹, Stefania Serra¹, Andrea Ranzi¹

¹Regional Agency for Prevention, Environment and Energy Emilia Romagna Region (Arpae), Bologna, Italy.

|April 23, 2024

概括

暴露于 perfluorooctane sulfonate (PFOS) 在体外诱导恶性细胞转化,而 perfluorooctanoic acid (PFOA) 没有. 这项研究使用了BALB/c 3T3细胞转化试验来评估PFAS的毒性.

科学领域:

环境毒理学环境毒理学
在体外毒理学.
致癌性研究的研究.

背景情况:

和多化基物质 (PFAS),包括PFOS和PFOA,是引起健康关注的持续性环境污染物.
关于PFOS/PFOA致癌性的流行病学数据不一致,动物基作用模式可能不适用于人类.

研究的目的:

用BALB/c 3T3细胞转化试验 (CTA) 来研究PFOS和PFOA在体外致癌潜力.
通过评估其非遗传毒性致癌潜力,为PFAS的监管评估做出贡献.

主要方法:

使用了BALB/c 3T3细胞转化试验 (CTA),这是一个用于非遗传毒性致癌物的体外模型.
根据经过验证的协议,将细胞暴露在各种度的PFOS和PFOA中.
评估细胞转化,细胞毒性和增殖诱导.

主要成果:

在度≥50 ppm的情况下,PFOS诱导了III型焦点的度依赖的增加,表明恶性转变.
在CTA试验中,PFOA没有诱导细胞转化.
PFOS诱导的转化与细胞毒性或扩散没有直接关联.

结论:

PFOS和PFOA在体外表现出不同的致癌特征,其中PFOS显示出恶性转变的潜力.
BALB/c 3T3 CTA可以区分PFOS和PFOA的转化潜力,这表明了不同的分子机制.
需要进一步的研究,以充分阐明PFOS和PFOA的毒理机制.

关键词:

在PFAS中,有很多方法.细胞转化试验细胞转化试验非遗传毒性的致癌性 perfluorooctane 硫酸硫酸的含量是什么 perfluorooctanoic 酸的使用情况.

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