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双A和三对体外T辅助细胞分化的单独和混合作用

Florence Fischer¹, Miriam Rebecca Ermer¹, Julia Howanski¹

¹Department of Environmental Immunology, Helmholtz Centre for Environmental Research GmbH - UFZ, Permoserstraße 15, 04318, Leipzig, Germany; Perinatal Immunology, Saxonian Incubator for Clinical Translation (SIKT), Medical Faculty, Leipzig University, Philipp-Rosenthal-Straße 55, 04103, Leipzig, Germany.

Chemico-biological interactions

|April 23, 2024

概括

干扰内分泌的化学物质 (EDC) 如双A (BPA) 和-3 (BP-3) 可以通过改变T助手 (TH) 17和调节性T (Treg) 细胞分化来破坏免疫平衡,混合物显示累积效应.

科学领域:

免疫学免疫学免疫学
内分泌学在内分泌学.
毒理学毒理学毒理学

背景情况:

免疫平衡对于病原体防御和自我容忍至关重要.
免疫平衡的障碍可能导致病理.
内分泌干扰化学物质 (EDC) 越来越多地与免疫失调有关.

研究的目的:

研究双A (BPA) 和-3 (BP-3),单独或组合,对T助手 (TH) 17和调控性T (Treg) 细胞分化的体外影响.
评估这些EDC及其混合物对T细胞子集的免疫毒性潜力.

主要方法:

从小鼠淋巴状组织中分离天真T细胞.
在不同度的BPA和BP-3的存在下,T细胞在体外分化成TH17和Treg群体.
分析细胞活力,增殖,细胞因子产生,转录因子表达 (流细胞计,CBA/ELISA) 和激素受体转录 (RT-PCR).

主要成果:

高度的BPA降低了TH细胞的活力和增殖.
暴露于EDC调节了TH17和Treg分化的中间状态,促进了TH1-样TH17细胞和TH2-样Treg细胞.
与单个化合物相比,BPA和BP-3的混合物表现出不同的效果,包括在TH17区分条件下降低雌激素受体β表达.

结论:

在体外,BPA和BP-3在T细胞分化的过程中显示出免疫干扰的潜力.
EDC混合物对T细胞分化的累积影响是显而易见的.
这些发现凸显了EDC与免疫系统调节之间的复杂相互作用.

关键词:

索芬-3的使用方法双 A 是一种双.化学混合物是一种化学混合物.内分泌干扰物干扰内分泌干扰物在体外分化中的差异化.监管T细胞监管T细胞一个TH17细胞.

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