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在升温期间补充脂肪酸改善了冷胚胎细胞移植后的妊娠结果:一项倾向性得分匹配研究

Ayano Sawado¹, Kenji Ezoe², Tetsuya Miki¹

¹Kato Ladies Clinic, 7-20-3 Nishishinjuku, Shinjyuku-ku, Tokyo, 160-0023, Japan.

Scientific reports

|April 23, 2024

概括

温暖溶液中的脂肪酸 (FA) 在体外显著改善了人体胚胎细胞活力和外生长. 这导致了更高的植入率,临床怀孕率和单次玻璃化加热胚胎细胞移植 (SVBT) 后的持续怀孕率.

科学领域:

生殖生物学生殖生物学
胚胎学胚胎学
辅助生殖技术辅助生殖技术

背景情况:

玻璃化和加热是辅助生殖技术 (ART) 的关键步骤.
在升温后优化胚胎细胞存活率和发育潜力对于成功的结果至关重要.
脂肪酸 (FA) 在细胞膜完整性和功能中起作用.

研究的目的:

评估脂肪酸 (FA) 补充加热溶液对人类胚胎细胞活性的影响,使用体外外生长模型.
通过使用FA补充溶液来评估单体玻璃化加热胚胎细胞移植 (SVBT) 后的怀孕结果.

主要方法:

使用FA补充或非补充 (对照) 溶液来加热废弃的人类胚芽细胞 (n=446).
通过测量外生长区域和退行发病率来评估体外外生长.
从1342名患者 (FA组) 的临床数据与2316名在自然循环中接受SVBT的对照进行了追溯分析.

主要成果:

用FA补充的溶液加热的胚芽细胞表现出显著更大的外生长区域 (P=0.0428).
在FA组中,外生长退化的发生率明显较低 (P=0.0158).
在SVBT后,FA补充剂显著增加了植入,临床怀孕和持续怀孕率 (P=0.0091-0.0266).

结论:

用脂肪酸补充的加热溶液在体外增强了人体胚胎细胞外生长潜力.
在单次玻璃化加热胚胎细胞移植后,FA补充改善了临床妊娠结果.
脂肪酸代表了改善胚胎囊冷保存和转移成功率的有希望的添加剂.

关键词:

胚胎囊细胞转移是什么意思脂肪酸是一种脂肪酸.在外生长的增长.怀孕的结果,玻璃化.

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