在黑睡鼠中发病率和脑肌心炎病毒-2的分子分析
Louise Gibson1, Tammy Shadbolt2,3, Pranab Paul3,4
1Institute of Zoology, Zoological Society of London, London, NW1 4RY, UK. Louise.gibson@ioz.ac.uk.
EcoHealth
|April 23, 2024
概括
脑肌心炎病毒-2 (EMCV-2) 已被检测到在英国的睡鼠种群下降. 这种病毒可能是特有的,并且可能与这些脆弱的哺乳动物的临床疾病有关.
科学领域:
- 兽医病毒学 兽医病毒学
- 野生动物疾病生态学 野生动物疾病生态学
- 保护生物学 保护生物学
背景情况:
- 英国的黑睡鼠 (Muscardinus avellanarius) 种群由于息地丧失而面临下降.
- 疾病监测对于识别对养殖和野生松鼠种群的威胁至关重要.
- 30多年的再引入计划并没有阻止国家衰退.
研究的目的:
- 为了调查死去的Hazel Dormice的死亡原因.
- 检测和描述影响休眠动物种群的新型或现有病毒威胁.
- 为了确定脑肌性心炎病毒-2 (EMCV-2) 在黑睡鼠中的患病率和潜在的临床意义.
主要方法:
- 下一代测序被用来识别已故的睡眠动物中的潜在病毒原体.
- 逆转录酶定量聚合酶连锁反应 (RT-qPCR) 和桑格测序被开发并用于检测EMCV-2RNA.
- 对阳性病例进行了甲胺固定组织的组织病理学检查.
主要成果:
- 一种与森林小鼠脑肌心炎病毒-2 (EMCV-2) 密切相关的病毒被确定为七只睡鼠的潜在死亡原因.
- 检测到的EMCV-2RNA是在44只被测试的黑动物中35只的肺组织样本中检测到的.
- 显微镜检查发现三例间歇性肺炎和心肌炎,尽管最终归因于EMCV-2受到潜在的辅因子的限制.
结论:
- 这项研究报告了首次检测到EMCV-2在黑睡中.
- 在英格兰的睡鼠种群中,EMCV-2很可能是特有的.
- 由于EMCV-2可能与临床疾病有关,因此需要进一步调查其对休眠动物健康和保护工作的影响.
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