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Updated: Jun 28, 2025

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
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Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

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在42例没有IDH体突变的质母细胞瘤的基因组景观:使用RNA测序数据进行全面分析

Takanari Okamoto1,2, Ryo Mizuta3,2, Yoshinobu Takahashi1

  • 1Department of Neurosurgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.

Journal of neuro-oncology
|April 23, 2024
PubMed
概括

这项研究使用RNA测序分析了42个质母细胞瘤的遗传突变,识别了TP53和EGFR变异. RNA测序是一种成本效益高的质母细胞瘤基因定型方法.

关键词:
融合基因是一个融合基因.基因表达 基因表达 基因表达质母细胞瘤 (glioblastoma) 是一个一个RNA序列的RNA序列.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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