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面包小麦的野生亲戚Triticum timopheevii的染色体规模基因组组合

Surbhi Grewal¹, Cai-Yun Yang², Duncan Scholefield²

¹Wheat Research Centre, Department of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Loughborough, LE12 5RD, UK. surbhi.grewal@nottingham.ac.uk.

Scientific data

|April 23, 2024

概括

研究人员生成了Triticum timopheevii的高质量基因组组,这是一种野生小麦的亲属. 该资源有助于发现改善小麦生产和确保全球粮食安全的基因.

科学领域:

植物基因组学植物基因组学
农作物科学农作物科学
生物信息学是一种生物信息学.

背景情况:

小麦 (Triticum aestivum) 是全球重要的粮食来源,面临着日益增长的需求.
提提 (Triticum timopheevii) 是一个全类野生亲属,它拥有宝贵的遗传资源,可以改善小麦.
高质量的参考基因组对于理解和增强作物特征至关重要.

研究的目的:

为了生成Triticum timopheevii加入PI 94760.0.的染色体尺度参考基因组组.
为识别农业学重要基因提供基础资源.
支持基因组信息化战略,以提高小麦生产和粮食安全.

主要方法:

利用PacBio HiFi测序进行高精度的长时间读取.
用于基因组架构的染色体构造捕获 (Hi-C).
进行了基因组注释和DNA甲基化分析.

主要成果:

产生了一个9.35 Gb的染色体尺度组件,具有42.4 Mb的N50结合.
包括线粒体和塑体基因组序列.
对166,325个基因模型进行了注释,其中包括70,365个高可信度基因;确定了A^t和G基因组之间的差异性DNA甲基化.

结论:

这种Triticum timopheevii基因组组合是一个重要的基因组资源.
促进发现改善小麦特征的基因.
有助于推进小麦育种计划和全球粮食安全.

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.