相关实验视频
Updated: Jun 28, 2025

11:26
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
13.7K
通过纳米孔测序检测嵌入在DNA中的核糖核酸的检测
Lavinia Grasso1, Adriano Fonzino2, Caterina Manzari2
1Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy.
Communications biology
|April 23, 2024
概括
这项研究表明,牛津纳米孔技术可以在DNA中直接检测核糖核酸 (rNMPs). 这一突破有助于理解基因组的不稳定性,并开发新的诊断工具.
科学领域:
- 基因组学和分子生物学
- 生物技术和生物信息学
背景情况:
- 核糖核酸 (rNMPs) 是真核基因组中常见的非正规核酸,但它们的起源和在基因组不稳定中的作用尚不清楚.
- 目前的测序方法间接识别基因组rNMP位置,限制直接分析.
- 未修复的rNMP与基因组不稳定性和人类疾病有关.
研究的目的:
- 研究牛津纳米孔技术 (ONT) 在基因组DNA中直接检测rNMP的潜力.
- 开发用于识别使用ONT原始测序信号的rNMP集成站点的方法.
- 为分析带有嵌入式核糖核酸的原生基因组奠定基础.
主要方法:
- 合成具有已知或随机位置的rNMPs的DNA分子.
- 为基于ONT的rNMP检测量身定制的数据分析管道的开发.
- 分析ONT原始信号特征,包括核酸对齐,电流强度和停留时间.
主要成果:
- ONT测序成功地识别了所有四种类型的核糖核酸,这些核糖核酸都被纳入了DNA.
- 通过识别由rNMPs引起的ONT信号特征的特定变化来实现检测.
- 这项研究表明,ONT能够直接绘制基因组DNA中的rNMP位点.
结论:
- 牛津纳米孔技术可以在基因组DNA中直接映射核糖核酸.
- 这项技术为研究rNMPs对基因组稳定性的影响提供了一种新的方法.
- 建议制定一种战略,用于使用rNMPs进行本地基因组分析的特设基调调器.
相关概念视频
RNA-seq
9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Sanger Sequencing
754.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.1K
Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
Next-generation Sequencing
88.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.7K

