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循环PHGDH降低调节通过miR-122-5p/PKM2轴减缓了乳头甲状腺癌的进展

Jiying Shen¹, Zhirong Ma¹, Jin Yang¹

¹Department of General Surgery, Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, 200336, Shanghai, China.

|April 23, 2024

概括

循环RNA糖酸脱酶 (circPHGDH) 促进皮皮质甲状腺癌 (PTC) 侵袭和转移. 下调circPHGDH通过影响miR-122-5p/PKM2通路和有氧糖解来抑制PTC进展.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

乳头甲状腺癌 (PTC) 侵袭和转移会影响患者的生活质量.
循环RNAs (circRNAs) 参与癌症调节,但它们在侵入性PTC中的作用尚未完全理解.

研究的目的:

为了研究circRNAs在侵袭性乳头甲状腺癌中的作用.
探索PTC中circRNA糖酸脱酶 (circPHGDH) 的表达,机制和功能.

主要方法:

下一代测序以识别异常的circRNA表达.
在PTC细胞系和组织中对circPHGDH进行定量分析.
在体外和体内测试 (Transwell,CCK8,EDU,转移,瘤发生) 来评估细胞迁移和增殖.
生物信息学和 luciferase 报告员测试以确定下游目标.

主要成果:

在PTC组织和细胞系中,circPHGDH表达升高,与PTC进展相关.
循环PHGDH的下调在体外和体内显著抑制了PTC细胞的入侵和增殖.
证实circPHGDH是针对microRNA (miR)-122-5p和pyruvate kinase M2亚型 (PKM2) 的目标.
过度表达PKM2或抑制miR-122-5p可以通过恢复有氧糖解来逆转circPHGDH沉默的抑制作用.

结论:

循环PHGDH下调抑制了PTC进展.
该机制涉及对miR-122-5p/PKM2轴的调节.
有氧糖解是PTC中circPHGDH调节途径的关键调解体.

关键词:

有氧糖溶解是有氧的.在PKM2中,PKM2是PKM2.乳头甲状腺癌是一种乳头甲状腺癌.环PHGDHDH 的时间在 miR-122-5p 中使用.

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