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水抗原对介质干细胞的免疫调节作用:基因表达变化和功能后果

Xin Wang¹, Wubulikasimu Mijiti¹, Zhifei Yi¹

¹Department of Orthopedics and Trauma, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang, China.

Frontiers in microbiology

|April 24, 2024

概括

酸性疾病抗原会改变细胞间干细胞 (MSC),减少它们的迁移并促进抗炎状态. 这种免疫调节可能有助于寄生虫逃避宿主防御,并在体内持续存在.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
寄生虫学的寄生虫学

背景情况:

囊性球菌,由 * Echinococcus granulosus * 引起,是一个重要的全球健康问题.
介酶干细胞 (MSC) 具有重要的再生和免疫调节功能.
MSCs和*Echinococcus granulosus*抗原之间的相互作用对于理解疾病的发病过程至关重要.

研究的目的:

为了研究 * Echinococcus granulosus * 抗原对MSC功能的影响.
为了确定MSCs如何对水类疾病衍生的抗原做出反应.
探索MSCs在寄生虫对宿主免疫逃避中的潜在作用.

主要方法:

从小鼠骨髓中分离出MSC,并与寄生虫抗原 (ESPs,HCF,PLL) 共培养.
使用高通量测序来分析MSC中的mRNA表达变化.
细胞周期,迁移和分泌功能通过CCK8,流细胞计,实时PCR,西斑和ELISA进行评估.

主要成果:

酸抗原显著改变了MSCmRNA表达,影响了细胞因子和化学因子的活性.
观察到一种转向抗炎性表型的转变,下调的促炎性细胞因子 (IL-6,NOS2/NO) 和上调的抗炎媒介 (COX2/PGE2).
所有测试的抗原减少了MSC迁移,细胞代谢途径显著改变.

结论:

* Echinococcus granulosus * 抗原诱导MSC的免疫调节反应,将其转移到具有减少迁移的抗炎类型.
这些MSC变化可能通过使免疫逃逸能够促进寄生虫的持久性.
这些发现为水虫疾病病理生理学和潜在的治疗点提供了洞察力.

关键词:

抗原是一种抗原.细胞因子细胞因子水病是水病的一种疾病.免疫调节免疫调节免疫调节中介细胞干细胞 (MSCs)

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