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优化SARS-CoV-2在95°C的热失活及其影响:一种标准化的方法
Gannon C K Mak1, Stephen S Y Lau1, Kitty K Y Wong1
1All from Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region.
Heliyon
|April 24, 2024
概括
针对SARS-CoV-2的标准化热失活程序对于质量评估至关重要. 一种10分钟的95°C无活化方法有效地准备了SARS-CoV-2外部质量评估计划样本,参与者的共识很高.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 缺乏针对SARS-CoV-2的标准化热失活协议.
- 现有的准备外部质量评估计划 (EQAP) 样本的方法在没有直接比较数据的情况下有所不同.
研究的目的:
- 评估和优化SARS-CoV-2的热失活程序.
- 为了确定SARS-CoV-2无活化的不同热处理持续时间的有效性.
主要方法:
- SARS-CoV-2 被放置在 95°C 的温度下,持续了 5,10 和 15 分钟.
- 通过缺乏细胞病变效应和在细胞培养通道上减少病毒载荷来证实失活的有效性.
- 为EQAP测试分配了优化的样本.
主要成果:
- 所有测试条件都使SARS-CoV-2无活化,显著降低病毒载量.
- 在10分钟的95°C条件下,产生了均且稳定的SARS-CoV-2 EQAP样本.
- 在发送的样本上,EQAP参与者表现出高度的同意.
结论:
- 评估的热失活条件可以帮助实验室准备SARS-CoV-2 EQAP样本.
- 10分钟的95°C处理对于生成可靠的SARS-CoV-2质量评估材料是有效的.
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