非线性弹性瓶刷 聚合物基调节 Actomyosin 中介突起形成 在介质细胞流体细胞中
Monica L Ohnsorg1,2, Kayla M Mash3, Alex Khang1,2
1Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, 80308, USA.
Advanced materials (Deerfield Beach, Fla.)
|April 24, 2024
概括
研究人员使用瓶聚合物开发了应变强化水凝,以模仿组织力学. 这些材料揭示了矩阵刚度如何影响人类介质细胞 (hMSCs) 和它们独特的形态.
科学领域:
- 生物材料科学 生物材料科学
- 细胞机械生物学 细胞机械生物学
- 聚合物化学 聚合物化学
背景情况:
- 组织细胞外矩阵表现出非线性弹性,其特点是应力强化和应力放松,这在合成生物材料中很难复制.
- 纤维结构通常需要模仿这些复杂的机械性能.
研究的目的:
- 为了合成和表征玻璃刷聚合物基的水凝,这些水凝表现出可调节的应变强化行为.
- 研究这些非线性弹性特性对人类介质细胞 (hMSC) 形态和行为的影响.
- 确定参与hMSC机械感知工程微环境的细胞机制.
主要方法:
- 合成基于聚乙烯糖醇的瓶刷聚合物.
- 交叉连接聚合物以形成具有控制网络架构的水凝.
- 聚合物长度的系统变化,以调整网络硬的关键应力.
- 3D拉力力显微镜测量细胞矩阵相互作用的数量.
- 用小分子抑制剂进行治疗,以探测细胞机械感知通路.
主要成果:
- 瓶刷聚合物水凝成功地重现了应变强化和应力放松的特性.
- 观察到一种独特的,突出丰富的hMSC形态,特别是在生物相关范围内的关键压力下.
- 通过抑制剂研究确定了参与机械感知的细胞机械.
结论:
- 联交联的瓶刷聚合物水凝可以有效地模仿本地组织的非线性生物机械线索.
- 这些工程材料提供了一个平台来研究矩阵非线性弹性如何调节细胞行为和机械感知.
- 这些发现凸显了矩阵应变强化的重要作用在指导细胞形态和功能的过程中.
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