对益生菌受体 (PRLR) 基因非同义单核酸多态 (nsSNP) 的全面的分析揭示了对蛋白质结构,功能和相互作用的多方面的影响
Anila Hoda1, Bajram Berisha2, Xhiliola Bixheku3
1Department Animal Sciences, Agricultural University of Tirana, Albania.
Journal of biomolecular structure & dynamics
|April 24, 2024
概括
这项研究在益生素受体 (PRLR) 基因中确定了有害的非同义单核酸多态 (nsSNP),揭示了它们对蛋白质稳定性,结构和功能的重大影响,这对于选择性繁殖至关重要.
科学领域:
- 遗传学和基因组学 在
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 益生素受体 (PRLR) 基因在包括哺乳在内的生物过程中起着至关重要的作用.
- 非同义单核酸多态 (nsSNP) 可以改变蛋白质的功能和结构,影响细胞过程.
研究的目的:
- 研究NSSNP在PRLR基因中的功能和结构后果.
- 识别有害的nsSNP并了解它们对选择性育种的分子和表型影响.
主要方法:
- 使用生物信息工具 (SIFT,Polyphen2,Panther) 来预测nsSNP的有害性.
- 使用DDG I-Mutant,DDG Mupro和CUPSAT评估了蛋白质的稳定性.
- 使用DynaMut和MutPred2.2进行结构分析.
- 使用了保护分析 (ConSurf) 和交互网络分析 (STRING).
- 进行了分子动力学 (MD) 模拟和翻译后修饰 (PTM) 分析.
主要成果:
- 确定了13种有害的nsSNP,其中Polyphen2和Panther预测的危害概率很高.
- 突变始终降低了蛋白质的稳定性,并诱导了结构变化,特别是在功能领域内.
- 观察到受影响的残留物高度保存,并参与与哺乳相关的途径.
- MD模拟揭示了结构动态,PTM分析确定了特定的修改点.
结论:
- 在PRLR基因中有害的nsSNP显著影响蛋白质的稳定性,结构和功能.
- 这些发现为了解PRLR相关特征和选择性育种策略提供了关键的分子见解.
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