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基于质谱学的伪向代谢学揭示了a2诱导的胃癌细胞中的代谢变异

Juan Li^1,2, Ying Liu¹, Piao Zhou¹

¹School of Pharmaceutical Sciences, Institute of Drug Discovery and Development, Zhengzhou University, Zhengzhou, China.

European journal of mass spectrometry (Chichester, England)

|April 24, 2024

概括

化合物a2,一个Jiyuan oridonin衍生物,显示了胃癌 (GC) 治疗的前景. 它显著改变了GC细胞中的代谢物,其中大多数是减少的,这表明GC的新疗法方法.

科学领域:

在瘤学瘤学.
代谢学代谢学代谢学
药理学药理学是指药理学的学科.

背景情况:

胃癌 (GC) 由于其高死亡率和发病率而构成重大全球健康挑战.
化合物a2,一种Jiyuan oridonin的衍生物,已经证明对GC细胞具有强大的抗增殖作用,表明其作为治疗剂的潜力.

研究的目的:

为了研究MGC-803胃癌细胞在与化合物a2.2治疗后的代谢变化.
探索细胞对a2化合物的反应,作为胃癌治疗的新型候选药物.

主要方法:

使用了伪准代谢学方法.
使用液体染色学-并联质谱法 (LC-MS/MS) 进行代谢物分析.
应用多变量统计分析来解释代谢变化.

主要成果:

化合物a2治疗诱导了MGC-803细胞的显著代谢变化.
受影响的关键代谢途径包括氨基酸-tRNA生物合成,氨酸,阿斯巴酸和谷氨酸代谢,胺代谢和三酸循环.
在低剂量和高剂量治疗组中,大约80%的已识别的代谢物,如阿斯巴酸盐,三,sedoheptulose 7-phosphate,succinate,2'-deoxyadenosine,uridine和cytidine的下调.

结论:

这项研究提供了化合物a2在胃癌治疗潜力的证据.
在用a2化合物治疗的GC细胞中观察到的代谢重编程支持其新的作用机制.
这些发现表明a2化合物是开发新的胃癌治疗方法的有希望的候选者.

关键词:

这是LC-MS/MS.在MGC-803细胞中.化合物 a2 化合物 a2 胃癌胃癌是一种胃癌.伪向的代谢学.

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