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在平面体外试验中对细胞毒性掩饰效应的推断

Timothy Rosenberger¹, Anna Maria Bell¹, Georg Reifferscheid¹

¹Department G - Qualitative Hydrology, Federal Institute of Hydrology (BfG), Am Mainzer Tor 1, 56068, Koblenz, Germany.

Analytical and bioanalytical chemistry

|April 24, 2024

概括

细胞毒性往往掩盖了体外测试中的效应,但用于平面生物测试的新峰值拟合方法量化了这些受影响的信号. 这种方法纠正了剂量反应数据,这对于准确的混合物效应建模至关重要.

科学领域:

环境化学环境化学
毒理学毒理学毒理学
分析化学分析化学

背景情况:

细胞毒性可以掩盖或减少体外测试中的效果信号,阻碍准确的量化.
现有的在常见的体外测试中识别细胞毒性掩盖的方法是有限的,不允许信号量化.

研究的目的:

调查使用平面生物试验的双峰的潜力,以量化细胞毒性影响的信号.
评估一个峰值适配方法,从受干扰的剂量反应数据中推断出理论的,未受影响的信号.

主要方法:

使用平面酵母雌激素查 (p-YES) 试验,将薄层染色学与体外试验相结合.
应用了四个个个别的峰值函数来建模42个理想的,从雌激素化合物中未扭曲的峰值.
分析了双A (BPA) 实验的细胞毒性扰乱信号和弹性材料的液样本.

主要成果:

峰值拟合方法成功实现了对细胞毒性影响信号的定量评估.
与未补偿数据相比,BPA的模拟ED50值是细胞毒性补偿的13倍.
该方法对混合物效应建模具有高度相关性,需要完整的剂量反应关系.

结论:

开发的拟合方法可以在平面体外试验中对细胞毒性影响的信号进行定量评估.
该方法适用于涉及扭曲色谱信号的其他化学分析领域.
准确量化受影响信号对于可靠的毒理学评估和混合物效应预测至关重要.

关键词:

染色体评价的评价染色体评价细胞毒性掩盖是细胞毒性的.效果推算效果推算方法平面在体外测定试验中的测定.量化量化量化量化

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