Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Balancing O-GlcNAc and O-fucose in plants.

The FEBS journal·2021
Same author

Author Correction: Light triggers PILS-dependent reduction in nuclear auxin signalling for growth transition.

Nature plants·2021
Same author

Retraction Note: PPP1, a plant-specific regulator of transcription controls Arabidopsis development and PIN expression.

Scientific reports·2021
Same author

Targeting alternative splicing by RNAi: from the differential impact on splice variants to triggering artificial pre-mRNA splicing.

Nucleic acids research·2021
Same author

A Novel, Broad-Acting Peptide Inhibitor of Double-Stranded DNA Virus Gene Expression and Replication.

Frontiers in microbiology·2020
Same author

The <i>Arabidopsis</i> O-fucosyltransferase SPINDLY regulates root hair patterning independently of gibberellin signaling.

Development (Cambridge, England)·2020

相关实验视频

Updated: May 11, 2026

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
07:59

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Published on: May 17, 2017

10.1K

使用共聚焦显微镜进行根毛成像.

Krishna Vasant Mutanwad1, Monika Debreczeny2, Doris Lucyshyn3

  • 1Institute of Molecular Plant Biology, Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.

Methods in molecular biology (Clifton, N.J.)
|April 24, 2024
PubMed
概括

研究植物遗传学揭示了控制根毛发发育的关键基因. 像显微镜和基因突变技术这样的技术有助于可视化Arabidopsis thaliana的根毛形态和细胞命运决定.

关键词:
阿拉比多普西斯塔利亚纳.同焦点显微镜的共聚焦显微镜光显微镜的光学显微镜.化染色 化染色根毛发形成的过程.

更多相关视频

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform
09:23

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

Published on: August 15, 2017

8.6K
RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

16.2K

相关实验视频

Last Updated: May 11, 2026

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
07:59

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Published on: May 17, 2017

10.1K
Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform
09:23

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

Published on: August 15, 2017

8.6K
RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

16.2K

科学领域:

  • 植物生物学 植物生物学
  • 遗传学 遗传学 是一个
  • 发展生物学 发展生物学

背景情况:

  • 植物根毛发的发育是一个复杂的过程,受到众多基因的影响.
  • 了解根毛形态和细胞命运对于植物生长和营养吸收至关重要.
  • 使用突变物和转基因线的遗传研究对于剖析这些过程至关重要.

研究的目的:

  • 详细介绍了可视化Arabidopsis thaliana根毛发发育和发育的方法.
  • 介绍观察根毛发分布和表皮细胞命运的技术.
  • 为植物根研究中使用显微镜和遗传工具提供指南.

主要方法:

  • 利用功能丧失突变物和过度表达的植物系来研究基因意义.
  • 采用光标记结构用于基因可视化.
  • 应用共聚焦激光扫描显微镜来捕获根发形态的高分辨率图像.
  • 开发用于成像YFP标记的转基因线路的方法,以追踪早期细胞命运.

主要成果:

  • 详细的成像技术用于根毛分布在Arabidopsis根的延长区域.
  • 成功的可视化早期的表皮细胞命运的决定在根的顶端干系统.
  • 证明共聚焦显微镜在记录根发特征方面的实用性.

结论:

  • 基因和微观方法为研究植物根的发育提供了强大的工具.
  • 描述的方法有助于研究控制根毛发表型和形态的基因.
  • 这项工作有助于更深入地了解植物根中的细胞过程.