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使用F₂和RIL/DH绘制人口基因地图的基因地图构造

Beata Myśków¹, Paweł Milczarski², Stefan Stojałowski²

¹Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin (ZUT), Szczecin, Poland. bmyskow@zut.edu.pl.

Methods in molecular biology (Clifton, N.J.)

|April 24, 2024

概括

本研究详细介绍了使用JoinMap5.0程序构建遗传地图的方法,概述了创建人口映射和分析定量特征的方法. 它为植物遗传学研究人员提供了实用指南.

科学领域:

遗传学是一个遗传学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

基因映射决定了染色体上的基因位置和距离.
绘制F2和RIL等种群的地图对于构建遗传地图至关重要.
双体 (DH) 种群是有价值的,但在某些作物中很难获得.

研究的目的:

介绍使用JoinMap5.0程序构建遗传地图的程序.
为了展示两种不同的算法用于遗传映射的使用.
引导研究人员获取人口映射和准备数据进行分析.

主要方法:

使用JoinMap5.0软件进行遗传地图构建.
采用来自映射种群 (例如,F2,RILs) 的基因类型数据.
应用多态标记器用于链接分析和定量特征分析.

主要成果:

提供了基因地图构建的详细协议.
展示了两个不同的映射算法的应用.
允许使用构造的遗传地图分析定量特征.

结论:

遗传地图是了解物种遗传学和定量特征的重要工具.
JoinMap5.0提供了一个强大的基因地图构建平台.
提出的方法有助于在植物育种和研究中进行遗传分析.

关键词:

基因型定制是指基因型定制.链接分析链接分析几率的对数 (LOD) 几率的对数最大的概率算法 (ML)分子制造者分子制造者回归映射算法 (RM) 是一种回归映射算法.

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