相关实验视频

Updated: Jun 28, 2025

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
05:29

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

Published on: June 9, 2021

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用头空间固相微提取 (HS-SPME) 与气色谱-质谱 (GC-MS) 结合使用番茄果实中挥发性物质的识别

Prateek Gupta1,2, Ajayakumar Jaya Dhanya1, Rameshwar Sharma1

  • 1Repository of Tomato Genomics Resources, Department of Plant Sciences, University of Hyderabad, Hyderabad, India.

Methods in molecular biology (Clifton, N.J.)
|April 24, 2024
PubMed
概括

这项研究提出了一种有效的方法来分析番茄果实中的植物挥发性有机化合物 (VOC). 头部空间固体相微提取-气色谱-质谱技术允许对这些重要的植物化合物的敏感和选择性提取和识别.

关键词:
气体染色学 是一种气体染色学.头部空间固体相微提取微提取质谱测量质量谱测量在SPME纤维纤维上.茄子 番茄子 番茄子挥发性有机化合物 挥发性有机化合物

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相关概念视频

Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
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