在沙特阿拉伯克罗恩病患者的肠道微生物组合
Hadil Alahdal1, Ghaida Almuneef1, Manal Muhammed Alkhulaifi2
1Department of Biology, Faculty of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
PloS one
|April 24, 2024
概括
克罗恩病 (CD) 与肠道微生物多样性减少有关. 吸烟,年龄老龄化和男性性别显著影响微生物群,突出了沙特阿拉伯疾病管理的潜在治疗目标.
科学领域:
- 胃肠病学 胃肠病学
- 微生物组研究 微生物组研究
- 免疫学 免疫学 免疫学
背景情况:
- 克罗恩病 (CD) 涉及与肠道微生物组合和多样性的复杂相互作用.
- 肠道微生物群在疾病发病的作用尚未完全被理解,特别是在沙特阿拉伯.
研究的目的:
- 在沙特阿拉伯描述肠道微生物群和克罗恩病之间的关系.
- 为了确定影响CD活动,微生物群失调和生活质量的因素.
主要方法:
- 高通量转基因16SrRNA基因测序 (V3-V4区域) 在80名CD患者 (活跃和不活跃) 和健康对照中进行.
- 统计分析将微生物数据与人口统计,生活方式 (问卷),诊断和治疗信息相关联.
- 用α-多样性测量来评估细菌的多样性和丰富性.
主要成果:
- 与对照组相比,在活跃的和不活跃的CD患者中观察到较低的细菌多样性和丰富性.
- 在活跃的CD患者中,显而易见的是更大的功能失调,标志着微生物多样性减少.
- 特定的细菌 (Filifactor,Peptoniphilus,Sellimonas) 与CD相关,而其他细菌 (Defluviitalea,Papillibacter,Petroclostridium) 与对照相关.
- 吸烟显著减少了阿尔法多样性和丰富性,增加了促炎性Fusobacteria.
- 高龄和男性性别与CD患者的微生物多样性较低有关.
结论:
- 这项研究提供了沙特阿拉伯克罗恩病患者肠道微生物群的第一个特征.
- 微生物群失生症是CD的关键特征,受到吸烟,年龄和性别等因素的影响.
- 这些发现表明,针对微生物组的向疗法有可能用于控制CD进展.
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