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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

57.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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PCR01:32

PCR

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Overview
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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相关实验视频

Updated: Jun 28, 2025

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
11:58

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

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放大时间R:用于定时连续放大事件的R包.

G Maria Jakobsdottir1,2, Stefan C Dentro3,2, Robert G Bristow1,4,2

  • 1Division of Cancer Sciences, University of Manchester, Manchester Academic Health Science Centre, Manchester M13 9PL, United Kingdom.

Bioinformatics (Oxford, England)
|April 24, 2024
PubMed
概括
此摘要是机器生成的。

AmplificationTimeR精确地乘以更高层次的副本数量增长,并推断基因组事件的顺序,扩展了定时焦点放大现有的方法.

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

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相关实验视频

Last Updated: Jun 28, 2025

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
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Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

Published on: June 25, 2014

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

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科学领域:

  • 基因组学就是基因组学.
  • 计算生物学 计算生物学
  • 癌症研究 癌症研究

背景情况:

  • 对于在焦点基因组区域中计时个别放大事件的方法有限.
  • 目前的技术在它们可以分析的副本编号状态中受到限制.

研究的目的:

  • 引入AmplificationTimeR,一种用于计时更高层次副本数量的新方法.
  • 推断基因组事件的最节的顺序,包括单个收益和全基因组重复.

主要方法:

  • 扩展时间R扩展了定时基因组获取的既定方法.
  • 该方法分析了单个增长和全基因组重复的区域.

主要成果:

  • AmplificationTimeR可以定时更广泛的副本编号状态.
  • 结果与重叠副本编号状态的现有方法相似.

结论:

  • AmplificationTimeR为分析复杂的基因组放大提供了一个强大的工具.
  • 研究人员可以免费使用R包.