用银离子激发单链和i-Motif DNA 的状态
Zakhar V Reveguk1, Evgeny V Khoroshilov2, Andrey V Sharkov2
1Department of Molecular Biophysics and Polymer Physics, Saint-Petersburg State University, Universitetskaya nab. 7/9 , 199034 St. Petersburg, Russia.
The journal of physical chemistry. B
|April 24, 2024
概括
银离子与细胞因子DNA链结合,形成由C-Ag+-C键稳定的复合体. 这种结合会改变DNA结构和激发状态属性,这取决于离子度和结合点.
科学领域:
- 生物物理化学 生物物理化学
- 分子生物物理学 分子生物物理学
- DNA DNA 纳米技术 纳米技术
背景情况:
- 富含细胞因子的DNA序列可以形成复杂的结构,如i-motifs.
- 已知银离子 (Ag+) 与DNA相互作用,影响其结构和特性.
- 了解这些相互作用对于开发基于DNA的新材料和治疗方法至关重要.
研究的目的:
- 研究与银离子 (Ag+) 复合的细胞因子 (dC) 10链的结构性和兴奋状态性质.
- 确定不同的Ag+度和pH对DNA复合体形成和稳定性的影响.
- 阐明Ag+与细胞因子相互作用的结合部位和机制.
主要方法:
- 循环二重化 (CD) 光谱学.循环二重化 (CD) 光谱学.
- 稳态吸收和光谱学.
- 超快的光向上转换光谱学.
- 垂直电子过渡能量的计算计算.
主要成果:
- (dC) 10个链通过C-Ag+-C键形成Ag+稳定双重链.
- (dC) 10的i-基因结构被Ag+离子的存在破坏了稳定.
- 兴奋状态属性由Ag+结合调节,在与细胞蛋白的O或N7原子相互作用时出现新的低层状态.
结论:
- 银离子诱导丰富的细胞因子DNA的显著结构变化,有利于双重组的形成,而不是i-motif结构.
- +离子的结合点和固态度决定了在激发状态动态中观察到的变化.
- 这些发现提供了对金属-DNA相互作用的见解,对DNA纳米技术和传感应用有意义.
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