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在Bordetella bronchiseptica犬种隔离物中对外膜蛋白和皮肤菌毒素进行结构和免疫学比较分析

Ji Young Jang¹, Myung Whan Oh², Chaeyeong Na³

¹Department of Infectious Disease, College of Veterinary Medicine, Seoul National University, Seoul, South Korea; Green Cross Veterinary Products Co., Ltd, Yongin, South Korea.

Veterinary immunology and immunopathology

|April 24, 2024

概括

这项研究开发了一种新的犬类博德氏菌疫苗,评估了外膜蛋白 (OMP) 和皮肤毒素 (DNT) 域3. 研究结果显示,结合这些 Bordetella bronchiseptica 成分会产生明显的免疫反应和潜在的副作用.

科学领域:

兽医免疫学兽医免疫学
疫苗开发疫苗开发
细菌病原体的产生

背景情况:

Bordetella bronchiseptica会导致哺乳动物的呼吸道感染,因此需要为狗提供更安全的疫苗.
目前的犬类Bordetella疫苗有副作用,推动了对子单位疫苗的研究.
人类疫苗开发提供了一个创建改进犬类波氏菌疫苗的模型.

研究的目的:

通过评估外膜蛋白 (OMP) 和皮肤毒素 (DNT) 开发一种新型犬科波尔代泰拉疫苗. 3. 领域.
评估来自B. bronchiseptica的OMP和DNT-3诱导的免疫性和细胞因子概况.
调查与联合抗原使用相关的潜在协同效应和风险.

主要方法:

在体分析中识别DNT域,选择域3作为抗原.
使用SDS-PAGE和西方涂料提取和描述OMP.
用OMPs和DNT-3刺激的DH82细胞中基因表达和细胞因子生产的评估.

主要成果:

OMPs诱导了Th1偏倚的免疫反应,而DNT-3促进了Th17和Th2反应.
组合的OMP和DNT-3对IL-23的产生产生了协同作用,这表明副作用的风险增加.
单独和组合观察到OMP和DNT-3的独特免疫特征.

结论:

临床前数据表明,OMP和DNT-3在Bordetella bronchiseptica中引起不同的免疫反应.
联合使用抗原可能会增加由于协同IL-23诱导而导致疫苗副作用的风险.
这项研究为为伴侣动物和人类开发下一代Bordetella疫苗提供了基础.

关键词:

Bb Bordetella 支气管传染病细胞因子细胞因子 DNT皮肤菌毒素DNT皮肤菌毒素在体内进行分析.OMPs是外膜蛋白质的外膜蛋白质.亚单位疫苗亚单位疫苗

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