在人类中,肠道表达特征和LEAP2, ghrelin及其常见受体GHSR的肝脏表达
Anders Englund1, Hannah Gilliam-Vigh1, Malte P Suppli1
1Center for Clinical Metabolic Research, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark.
Peptides
|April 24, 2024
概括
在人体肠道和肝脏中研究了肝脏表达的抗微生物2 (LEAP2) 和 ghrelin mRNA表达. 没有发现与2型糖尿病或肥胖相关的显著差异.
科学领域:
- 内分泌学 在内分泌学.
- 胃肠病学 胃肠病学
- 代谢疾病 代谢疾病
背景情况:
- 肝脏表达的抗微生物2 (LEAP2) 和格林是对生长激素分泌受体 (GHSR) 有互惠作用的激素.
- LEAP2和ghrelin都与肥胖和2型糖尿病 (T2D) 的病理生理学有关.
- 了解它们在人类胃肠道和肝脏中的表达模式对于阐明它们在代谢健康中的作用至关重要.
研究的目的:
- 为了研究LEAP2,ghrelin和GHSR在人类肠道沿线的mRNA表达特征,在患有和没有T2D的个体中.
- 为了检查LEAP2和GHSR在肥胖和没有肥胖的男性肝脏中的mRNA表达.
主要方法:
- 大量转录组学分析是在T2D患者和健康对照者的小肠和大肠的粘膜活检上进行的.
- 从肥胖和瘦男人的肝脏活检也使用批量转录组学分析.
- 量化了LEAP2,ghrelin和GHSRmRNA的表达水平.
主要成果:
- 无论T2D状态如何,LEAP2mRNA表达在小肠中增加,在大肠门下降,在大肠中表达最小.
- 格雷林mRNA表达在十二指肠中强大,沿小肠下降,在大肠中表达很少,没有T2D相关的变化.
- 肝脏中LEAP2mRNA的表达很高,而肝脏活检中没有检测到格林和GHSRmRNA. 在肥胖和瘦男之间没有观察到肝脏LEAP2表达的差异.
结论:
- 在人类肠道沿线建立了LEAP2,ghrelin和GHSR的独特mRNA表达配置文件.
- 这些基因表达的显著变化与肠道2型糖尿病无关.
- 肝脏LEAP2表达在肥胖男性和瘦男性之间没有差异,这表明该组织与肥胖没有直接联系.
更多相关视频
09:16Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
Published on: April 20, 2017
15.5K
07:00Mechanisms Underlying Gut Hormone Secretion Using the Isolated Perfused Rat Small Intestine
Published on: February 26, 2019
9.5K
相关概念视频
Regulation of Food Intake
225
Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
225
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Glucagon-like Receptor Agonists
319
Incretins include glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), which stimulate insulin secretion post-meals. In type 2 diabetes, GIP's efficacy is reduced, making GLP-1 a viable drug target. GIP originates from preproGIP.
GLP-1, when administered in high doses intravenously, triggers insulin secretion, inhibits glucagon release, slows gastric emptying, reduces food intake, and restores normal insulin secretion. However, its rapid inactivation by...
GLP-1, when administered in high doses intravenously, triggers insulin secretion, inhibits glucagon release, slows gastric emptying, reduces food intake, and restores normal insulin secretion. However, its rapid inactivation by...
319
Hormonal Regulation
43.5K
Hormones regulate a significant portion of digestion through activation of the neuroendocrine system. The neuroendocrine system of digestion contains many different hormones all with multiple functions that are both, directly and indirectly, involved in digestion.
43.5K
Hormones Secreted by the Stomach
543
Enteroendocrine cells, accounting for only 1% of stomach epithelial cells, play a significant role in digestion and are classified by their digestive hormone secretions.
Each of these hormones secreted by different enteroendocrine cells plays a unique role in digestion. Here are a few examples:
Each of these hormones secreted by different enteroendocrine cells plays a unique role in digestion. Here are a few examples:
543
GPCRs Regulate Adenylyl Cylase Activity
5.5K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.5K
