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Updated: Jun 28, 2025

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用于铁进口的细菌跨膜蛋白EfeU的表达,净化和初步晶体分析

Kenji Okumura1, Bunzo Mikami2, Sayoko Oiki1

  • 1Laboratory of Basic and Applied Molecular Biotechnology, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Japan.

Protein expression and purification
|April 24, 2024
PubMed
概括

研究人员净化了EfeU蛋白,这是细菌铁 (Fe2+) 进口系统的关键组成部分. 已建立的方法为确定EfeU结构铺平了道路,这对于了解细胞铁吸收至关重要.

关键词:
结晶化 结晶化 结晶化有效的效果是什么?影响力 影响力埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.钢铁进口商 钢铁进口商它是独立于 siderophore 的.

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The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
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