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Updated: May 10, 2026

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
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从哺乳动物悬浮培养物中表达和净化化蛋白质

Miranda E Schene1, Daniel T Infield1, Christopher A Ahern1

  • 1Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA, USA.

Methods in enzymology
|April 24, 2024
PubMed
概括

这项研究表明,使用进化的tRNA和合成酶对与非正规氨基酸一起大规模生产真核蛋白. 这种方法可以为各种下游应用程序进行可扩展的蛋白质净化.

关键词:
简介:真核生物悬浮细胞.有的蛋白质是有的蛋白质.重组蛋白质净化再组合蛋白质净化

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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...