[CRISPR/Cas9技术在米育种应用中的实验教学设计]
Yaping Liu1, Bojun Ma1, Xifeng Chen1
1National Experimental Teaching Demonstration Center of Biology, College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
概括
这项研究为大学生介绍了一项新的CRISPR/Cas9基因编辑实验,以增强大米对细菌病菌的抵抗力. 实践方法加深了对分子育种的理解和实践技能.
科学领域:
- 农业科学 农业科学
- 生物技术是生物技术.
- 遗传学 是一个遗传学.
背景情况:
- 基因编辑CRISPR/Cas9是一种强大的作物改进工具.
- 教育计划需要整合先进的生物技术,如CRISPR/Cas9.
- 开发基因编辑的实用技能对于未来的科学家来说至关重要.
研究的目的:
- 用CRISPR/Cas9技术为大学生设计一个创新的教学实验.
- 通过基因编辑来增强大米植物对细菌病的抵抗力.
- 为学生提供分子育种技术的实践经验.
主要方法:
- 利用CRISPR/Cas9基因编辑来准对大米细菌疹易感性负责的基因.
- 在大米中开发CRISPR/Cas9应用的实用实验室协议.
- 在本科教学课程中实施实验.
主要成果:
- 学生获得了对CRISPR/Cas9原则和协议的更深入的理解.
- 学生获得了应用基因编辑用于作物改进的实践技能.
- 该实验成功地证明了增强抗米疾病的潜力.
结论:
- 设计的CRISPR/Cas9实验有效地将先进技术整合到生物教育中.
- 这种方法促进了实验教学中的科学研究和创新.
- 掌握CRISPR/Cas9技术为学生在农业领域的研发做好了准备.
更多相关视频
07:43Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
12.4K
06:37Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
Published on: July 1, 2021
4.5K
相关概念视频
01:59Plant Breeding and Biotechnology
18.9K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
18.9K
01:59CRISPR
50.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.8K
02:53CRISPR and crRNAs
17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K