使用实验设计,对益生菌菌株Lactobacillus paracasei IMC 502®的发酵进行纯素级介质成分选和度优化
Darshankumar Parecha1, Alberto Alfano1, Donatella Cimini2
1U niversity of Campania Luigi Vanvitelli, Department of Experimental Medicine, 80138 Naples, Italy.
Journal of industrial microbiology & biotechnology
|April 24, 2024
概括
优化的纯素生长介质显著提高了乳杆菌paracasei IMC502®生物量65%. 这项研究开发了一种具有成本效益的植物基媒介,用于增强益生菌的生产,避免与食物来源的竞争.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 乳酸菌 (Lactobacillus paracasei) IMC502®是一种广泛使用的益生菌.
- 优化其生长媒介对于高效的生物质生产至关重要.
- 当前的媒体可能不具有成本效益或可持续性.
研究的目的:
- 设计和优化一个纯素生长媒介的乳杆菌paracasei IMC502®.
- 通过使用植物成分来增强益生菌生物质生产.
- 为益生菌种植确定具有成本效益和可持续的营养来源.
主要方法:
- 实验设计 (DoE) 用于媒介优化.
- 评估了各种素食碳来源和植物性 (例如 guar peptone).
- 生物质生产与标准的德曼-罗戈萨-夏普 (MRS) 介质进行了比较.
主要成果:
- 与MRS相比,一个为素食主义者优化的媒介增加了Lactobacillus paracasei IMC502®生物量约65%.
- 优化的介质实现了94.11%的模型精度,R2和调整后的R2值超过95%.
- 发现MRS中的大多数盐和微量元素对于生物质生产来说不是必不可少的,因此可以进行中等简化.
结论:
- 一种新的,为素食主义者优化的生长介质显著提高了Lactobacillus paracasei IMC502®生物质产量.
- 瓜尔是一种可行的源,有可能降低成本,避免与粮食资源的竞争.
- 这种方法为高密度益生菌种植提供了一种可持续和成本效益的方法,适用于其他益生菌菌株.
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