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Updated: Jun 28, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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CoCas9是一种来自人类微生物组的紧核酶,用于高效和精确的基因组编辑

Eleonora Pedrazzoli1, Michele Demozzi1, Elisabetta Visentin1

  • 1Department of Computational, Cellular and Integrative Biology (CIBIO), University of Trento, 38123, Trento, Italy.

Nature communications
|April 24, 2024
PubMed
概括

科学家们从人类微生物组中发现了CoCas9,一个较小,高度活跃的CRISPR-Cas9核酶. 这种新工具推进了基因疾病治疗的基因编辑,并成功地用于小鼠的体内编辑.

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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