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骨髓瘤中circ_0076684的升调通过介导miRNAs/CUX1促进恶性过程

Pengfei Cai¹, Xin Fu¹, Xiaofei Li²

¹Department of Orthopeadics, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, No. 365 Renmin East Road, Jinhua City, 321000, Zhejiang Province, China.

Journal of orthopaedic surgery and research

|April 24, 2024

概括

循环RNAs (circRNAs) 促进骨髓瘤 (OS) 的进展. Circ_0076684,一个RUNX2衍生的circRNA,通过海绵化miRNA和增加Cut Like Homeobox 1 (CUX1) 表达来增强OS.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

循环RNAs (circRNAs) 是人类癌症发展的关键调节者.
骨髓瘤 (OS) 是一种具有复杂分子基础的原发性骨恶性瘤.
RUNX家族转录因子2 (RUNX2) 基因位点与OS病原发生有关.

研究的目的:

研究三种RUNX2衍生的circRNAs (circ_0076684,circ_0003563,circ_0076691) 在骨髓瘤中的作用.
确定这些circRNAs的调节机制和临床意义.
阐明circ_0076684对OS进展的功能影响.

主要方法:

定量实时PCR用于评估circRNA和mRNA的表达.
生物信息分析和功能实验 (体外和体内).
MiRNA海绵测试和目标基因验证.

主要成果:

在OS中,circ_0076684,circ_0003563,circ_0076691和RUNX2mRNA的表达受到上调,由CBX4.4驱动.
Circ_0076684表达与OS临床特征和患者预后相关.
Circ_0076684通过海绵化miR-370-3p,miR-140-3p和miR-193a-5p促进OS进展,导致CUX1表达的增加.

结论:

通过CBX4介导的转录激活可在OS中调节RUNX2衍生的circRNA和RUNX2mRNA.
在OS中,circ_0076684作为致癌性circRNA的功能.
电路_0076684通过miR-370-3p/miR-140-3p/miR-193a-5p/CUX1轴促进OS的进展.

关键词:

在 CUX1 的位置上,可以看到 CUX1 的位置.循环RNAs是一种循环RNA.骨质肉瘤骨质肉瘤是什么在RUNX2中使用RUNX2.这就是miRNAs.

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