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在体外研究酸盐和酸盐与谷氨酸之间的竞争反应

Canan Höçük Özkan¹, Mehmet Atakay², Bekir Salih²

¹Department of Chemistry, Faculty of Arts and Science, Middle East Technical University, Ankara, Turkey.

Journal of mass spectrometry : JMS

|April 25, 2024

概括

这项研究揭示了和如何与谷氨 (GSH) 相互作用. (IV) 破坏了 (III) 与GSH的结合,对GSH表现出更高的亲和力,并影响了复合物的稳定性.

科学领域:

环境化学环境化学
生物化学生物化学
毒理学毒理学毒理学

背景情况:

暴露会增加活性氧物种 (ROS),而会减轻ROS和重金属的影响.
以前的研究探讨了植物中和的相互作用,但它们与谷氨 (GSH) 的结合机制仍未得到研究.

研究的目的:

研究 (As) 和 (Se) 对它们与GSH结合机制的对抗性或协同作用.
在不同的pH值 (3.0,5.0和6.5) 检查这些相互作用.

主要方法:

使用液体色谱二极管阵列探测器/电子喷雾质谱 (LC-DAD/MS) 和LC-ESI-MS/MS.
分析了二进制系统 (Se(SG) 2 + As(III) 和 As(SG) 3 + Se(IV) 和一个三进制系统 (As(III) + Se(IV) + GSH).
在检查的系统中,As和Se的比率有所变化.

主要成果:

(III) 没有影响-氨 (Se(SG) 2) 复合物的稳定性.
(IV) 敌对地破坏了-氨 (As(SG) 3复合体,形成了Se(SG) 2.
在所有测试的pH值中, (IV) 对GSH的亲和力比 (III) 高.
谷三硫化物 (GSSSG) 被确定为二进制和三进制系统中的副产品.

结论:

与 (III) 相比, (IV) 对GSH具有更强的结合亲和力.
和与GSH之间的相互作用依赖于pH值,可能导致复杂的干扰.
了解这些相互作用对于管理和的毒性至关重要.

关键词:

在 LC-DAD/MS 中.亚是一种.三甲三甲是一种.是一种.单二-谷氨氨酸是一种单二-谷氨.

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