在囊性纤维化患者获得的气道培养物中研究pH调节的SERS微传感器
William H Skinner1, Nicola Robinson2, Gareth R Hardisty2
1EaStCHEM School of Chemistry, The University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3FJ, U.K.
ACS sensors
|April 25, 2024
概括
表面增强的拉曼散射微传感器 (SERS-MS) 提供了一种用于测量气道表面液体pH的新方法,用于囊性纤维化模型. 这项技术允许在呼吸系统研究中进行非侵入性pH监测和CFTR功能的评估.
科学领域:
- 生物医学工程 生物医学工程
- 呼吸系统生理学 呼吸系统生理学
- 纳米技术 纳米技术
背景情况:
- 空气道表面液体 (ASL) 酸化与囊性纤维化 (CF) 病理学有关.
- 在ASL中进行非侵入性pH测量是具有挑战性的,因为其体积较小,并且靠近气道上皮.
研究的目的:
- 开发和验证表面增强的拉曼散射微传感器 (SERS-MS) 用于测量ASL pH.
- 在人体呼吸道模型中研究跨表皮的pH梯度和CFTR功能.
主要方法:
- 使用SERS-MS与一个4-mercaptobenzoic acid (MBA) pH记者分子.
- 采用空气液体接口 (ALI) 培养物来建模呼吸道上皮质.
- 在ASL和基侧介质中测量pH值,并在CFTR刺激时量化pH值变化.
主要成果:
- 在ALI培养物中,SERS-MS成功测量了跨表皮的pH梯度.
- 在刺激囊性纤维化跨膜导电调节器 (CFTR) 后,量化ASL pH 变化.
- 评估了CFTR突变对CF和非CF供体衍生ALI培养的基线ASLpH的影响.
结论:
- 在CF研究中,SERS-MS为非侵入性ASL pH测量提供了可行的工具.
- 这项技术可以阐明ASL pH失调在CF病变发生过程中的作用.
- 通过SERS-MS,可以研究CFTR功能及其对气道表面pH的影响.
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