将散装糖转化为功能纤维:发现和应用一个热稳定的β-1,3-Oligoglucan酸化酶
Marc De Doncker1, Sofie Vleminckx1, Jorick Franceus1
1Department of Biotechnology, Centre for Synthetic Biology (CSB), Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Journal of agricultural and food chemistry
|April 25, 2024
概括
研究人员发现了一种新的酶,来自*Anaerolinea thermophila* (AtβOGP) 的β-1,3-oligoglucan phosphorylase,用于从糖中高分子质量β-1,3-glucans的经济高效生产. 这一突破使得功能纤维的可扩展合成成为可能.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 碳水化合物化学 碳水化合物化学
背景情况:
- 目前生产β-1,3-葡萄糖是昂贵的,产生异质的产品,限制其工业应用.
- 甘氨酸酸化酶提供区域选择性合成,但需要昂贵的原始基质,并产生短的寡糖.
- 需要可扩展,具有成本效益的方法来生产具有受控分子质量的β-1,3-葡萄糖.
研究的目的:
- 发现和描述用于高效β-1,3-葡萄糖合成的新型酶.
- 开发一种可扩展和经济的工艺,用于生产可切割的β-1,3-基糖.
- 克服β-1,3-葡萄糖的现有酶合成方法的局限性.
主要方法:
- 发现和描述一个来自*Anaerolinea thermophila**的温稳β-1,3-oligoglucan酸化酶 (*At*βOGP).
- 评估使用葡萄糖作为原始基质的*At*βOGP的催化效率.
- 将*At*βOGP与热稳定的糖酸酶合起来,以从糖中产生单合成.
主要成果:
- *At*βOGP表现出高的催化效率,热稳定性,并合成高分子量β-1,3-葡萄糖 (高达DP 75).
- 结合的酶系统实现了将糖糖几乎完全转化 (>99mol %) 成为可切割的β-1,3-基糖.
- 建立了一种具有成本效益的工艺,用于将散装糖转化为功能性β-1,3-葡萄糖.
结论:
- 新型的*At*βOGP酶和结合的酶过程使β-1,3-葡萄糖的可扩展和经济的生产成为可能.
- 这一进步解决了β-1,3-葡萄糖合成中的关键瓶,为更广泛的工业用途铺平了道路.
- 开发的方法有助于将可再生资源转化为有价值的功能纤维.
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