缓慢冷冷保存韩国牛肉胚芽细胞,并加上糖糖前平衡步骤
Seungki Jung1,2, Hyeonseok Sul2, Dongjin Oh1,3
1Veterinary Medical Center and College of Veterinary Medicine, Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Chungbuk National University, Cheongju, Republic of Korea.
Frontiers in veterinary science
|April 25, 2024
概括
在慢速冷之前向牛胚胎添加糖,可以显著提高存活率和化率. 这一脱水步骤有助于克服冰晶损伤,提高冷保存的效率,以保护遗传资源.
科学领域:
- 生殖生物学和冷保存科学.
- 牲畜遗传学和生殖细胞的保存.
背景情况:
- 胚胎冷保存对于遗传资源的保存至关重要,但生存率因方法而异.
- 在冷过程中形成冰晶可能会损害胚胎,降低其生存能力.
- 优化冷保存技术对于成功长期存储牛遗传物质至关重要.
研究的目的:
- 为了评估一种修改后的缓慢冷方法的效率,该方法包含使用糖糖的脱水步骤.
- 为了确定最佳的糖度,以改善牛胚胎在解后的存活率和活力.
- 评估砂糖预处理对胚胎完整性和细胞压力标志物的影响.
主要方法:
- 韩国本地牛卵细胞在体外受精并培养到胚芽细胞阶段.
- 在缓慢结之前,胚胎囊被预平衡,使用不同度的糖糖 (0,0.1,0.25,0.5M) 缓慢结.
- 在解后分析了生存率,化率,TUNEL测定和基因表达 (BCL2,HSP70,AQP3).
主要成果:
- 与对照组相比,在0.1M糖组观察到明显更高的胚胎存活率 (p<0.05).
- 在0.25M和0.5M糖群中,72小时后化率显著改善 (p<0.05).
- 0.25M糖组显示TUNEL阳性细胞减少和与亡和压力相关的基因表达改变.
结论:
- 使用0.25M糖的预处理显著提高了牛胚胎在缓慢冷和解后的生存能力.
- 通过减少冰晶的形成,以糖为媒介的脱水有效地减轻了冷伤害.
- 这种修改后的冷保存技术提供了一种有前途的方法,可以改善牛的生殖细胞质的保存.
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