儿童肺部疾病 - - 我们的诊断是否正确?
Priya Rajendran1, Silla Varghese Thomas1, Sarath Balaji2
1ICMR-National Institute for Research in TB, Chennai, India.
Frontiers in pediatrics
|April 25, 2024
概括
在儿童中诊断结核病 (TB) 是一个挑战. 实时多重PCR测试可以检测结核病和其他呼吸道病原体,有助于准确诊断儿科肺炎.
科学领域:
- 儿童传染病 儿童传染病
- 微生物学 微生物学
- 分子诊断学 分子诊断学
背景情况:
- 在儿童中细菌/病毒性肺炎和结核病 (TB) 的差异诊断是复杂的,因为微生物学确认存在困难.
- 儿童肺结核诊断需要改进的方法来准确识别致病原体.
研究的目的:
- 评估实时多重PCR试验的有效性,以诊断肺结核儿童的呼吸道病原体.
- 评估多重PCR在儿童患者的结核病与其他常见呼吸道感染区分方面的有用性.
主要方法:
- 分析了来自3至12岁儿童的185个呼吸道样本.
- 样品经过N-乙L-氨酸 (NALC) NaOH治疗,通过涂抹显微镜,Xpert® MTB/RIF和液体培养检测Mycobacterium结核病 (MTB).
- 多重实时PCR在经过处理的唾液上进行了细菌和真菌病原体的检测.
主要成果:
- 在185个样本中,有20个为MTB阳性.
- 在MTB阳性样本中,15个样本还显示了与细菌或真菌病原体的共感染.
- 145 (87%) 的MTB阴性样本对其他细菌或真菌病原体呈阳性.
结论:
- 结核病在儿童中可以与其他呼吸道病原体共存.
- 像多重PCR这样的同时检测方法对于诊断儿科结核病和肺炎非常有价值.
- 需要进行大规模的多地理研究来证实这些快速诊断测试的有用性.
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