在其原生宿主中Mycobacterium tuberculosis RecA 蛋白质的条件蛋白质拼接
Ryan F Schneider1, Kelly Hallstrom2, Christopher DeMott2
1Biomedical Sciences Department, School of Public Health, State University of New York at Albany.
bioRxiv : the preprint server for biology
|April 25, 2024
概括
环境因素,如DNA损伤和铜的影响ReCA在Mycobacterium结核病中的整体拼接. 这表明互联拼接可能是Mtb的一种新的监管机制.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 整蛋白是蛋白质部分,它们从前体蛋白质中分离出来,产生功能性蛋白质.
- 益因的作用,无论是自私的遗传元素还是有益的宿主因素,仍在争论中.
- 了解内部与宿主之间的相互作用至关重要,特别是在本地细菌环境中.
研究的目的:
- 调查环境对Mtb.内本地ReCA合的环境影响.
- 阐明 RecA intein及其产品的加工和降解途径.
- 探讨在Mtb.中互联拼接的潜在监管作用.
主要方法:
- 利用西方污点来分析蛋白质水平.
- 雇佣的促进者报告员测试以评估基因活性.
- 进行了涉及DNA破坏剂,铜处理和不同氧气条件 (低氧/正常氧) 的实验.
- 在Mtb和E. coli中进行了宫外表达研究.
主要成果:
- 在低氧条件下,DNA损伤剂和铜增强了RecA中蛋白拼接.
- 拼接 RecA 被 Mtb 蛋白质组处理;自由的整蛋白被快速降解.
- 在本地mtb中没有检测到未分离的前体蛋白,与大肠杆菌的积累形成鲜明对比.
- 在某些条件下,可以产生自由的N-extein,激活大肠杆菌中的LexA.
结论:
- 细菌环境条件显著调节RECA在Mtb中的拼接.
- 在本地宿主中,因特因拼接对于准确的生物学解释至关重要.
- 在Mtb.内,Intine拼接呈现出一种潜在的新型调节机制.
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