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描述和调节免疫细胞在狗脊椎间盘的促炎作用

Mary K Heimann¹, Kelly Thompson², Gilian Gunsch³

¹Department of Biomedical Engineering The Ohio State University Columbus Ohio USA.

|April 25, 2024

概括

退化的狗椎间盘显示杆细胞和巨细胞增加,类似于人类的腰部疼痛. 乳腺细胞驱动炎症,但克罗莫林和PAR2抗体显示出治疗潜力.

科学领域:

兽医医学兽医医学兽医医学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

椎间盘 (IVD) 退化是人类和狗的腰部疼痛的常见原因.
巨细胞 (MC) 和巨细胞 (MØ) 透与人类和动物IVD退化 (IVDD) 有关,但在犬类中尚未研究.
微粒细胞降粒触发炎症并激活IVD细胞上的蛋白酶激活受体2 (PAR2).

研究的目的:

在退化的犬类IVD中表征病理生理变化.
研究与犬核脉细胞 (NP) 共同培养的MCs的炎症作用.
评估建筑硬度对NP和MC的影响,并确定IVDD的潜在治疗方法.

主要方法:

从健康和椎间盘的犬体IVD组织中分析了形态,蛋白质和炎症标志物.
健康的NP细胞与MCS共同培养在糖结构中,用克罗莫林 (CS) 和PAR2抗剂 (PAR2A) 进行治疗.
评估了基因表达,硫化糖氨基甘油含量和结构度.

主要成果:

与健康组织相比,退化的犬类IVD显示血管增加 (CD 31+),MC三酶和MØs (CD 163+).
狗NP细胞与MCs共同培养上调了促炎基因,而更硬的微环境加剧了这些影响.
CS和PAR2抑制剂减少了狗NP细胞中的关键的促炎标志物.

结论:

退化的犬类IVD表现出增加的MCs,MØs和血管化,反映了人类的IVDD和LBP.
与犬类NP细胞共同培养的MCs促进炎症;CS和PAR2A显示为IVDD体外治疗药物的潜力.
这项研究提供了对犬类IVDD病理生理学和潜在治疗策略的见解.

关键词:

狗类犬类动物狗类犬类克罗莫林的酸盐退化退化退化椎间盘间的椎间盘之间腰部疼痛腰部疼痛腰部疼痛巨细胞是一个巨细胞.巨细胞巨细胞是什么蛋白质酶激活受体 2 的受体.

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