FOXP3全长拼接变体与脏异体移植耐受性相关
Qais W Saleh1,2, Afsaneh Mohammadnejad3, Martin Tepel1,2
1Department of Nephrology, Odense University Hospital, Odense, Denmark.
Frontiers in immunology
|April 25, 2024
概括
在移植后早期血液中,低水平的叉头盒P3 (FOXP3) 全长 (FOXP3fl) 拼接变体预测了移植功能的下降. 这一发现可能有助于识别患有渐进性全移植损伤风险的患者.
科学领域:
- 免疫学 免疫学 免疫学
- 移植生物学 移植生物学
- 分子生物学分子生物学
背景情况:
- 异构移植功能障碍是造成 premature 移植损失的主要原因.
- 叉头盒P3 (FOXP3) 对于T调节细胞功能和保持自我耐受性至关重要.
- 调查FOXP3拼接变体可能会揭示移植损伤的早期指标.
研究的目的:
- 测试外围血液中降低的FOXP3 mRNA拼接变体水平是否与移植后的渐进性全移植损伤相关.
- 识别早期生物标志物来预测脏全移植衰退.
主要方法:
- 在术后1日和29日从333名移植患者收集血液样本.
- 用于测量FOXP3拼接变体的转录的定量PCR,包括FOXP3全长 (FOXP3fl).
- 后勤回归分析了FOXP3水平与估计的淋巴细胞过率 (eGFR) 降低 (>5ml/min/1.73m2) 之间的关联.
主要成果:
- 与稳定的eGFR (p=0.02) 相比,eGFR下降的受体显示出明显较低的FOXP3fl水平.
- 多变量分析证实了较低的FOXP3fl和下降的eGFR (OR0.51,p=0.02) 之间的联系.
- 移植后第一天较低的FOXP3fl水平与更高的EGFR下降率 (p=0.04) 有关.
结论:
- 在术后第一天检测到的FOXP3fl拼接变异水平的降低与移植接受者显著的eGFR下降有关.
- 这些发现表明FOXP3fl可能作为严重的全移植损伤的早期预测生物标志物.
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