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内源细胞壁降解酶LytD对于Bacillus subtilis的生物控制活性很重要

Luotao Wang¹, Jianquan Huang², Si Chen¹

¹Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.

Frontiers in plant science

|April 25, 2024

概括

在Bacillus subtilis中Autolysin LytD对于葡萄菌的生物控制至关重要. 破坏LytD减少了病原体抑制和植物防御刺激,影响生物膜形成和殖民.

科学领域:

微生物学微生物学
植物病理学植物病理学
生物技术是生物技术.

背景情况:

自杀酶是细菌细胞壁降解酶 (CWDEs),对于糖体重塑至关重要.
细菌细菌至少拥有35个自解酶基因,但它们的功能复杂且往往多余.
细菌细菌 GLB191 是一种已知的生物控制剂,用于对抗葡萄菌,直接作用于病原体并刺激植物防御.

研究的目的:

调查自溶酶N-乙糖氨酸酶LytD在Bacillus subtilis对葡萄菌的生物控制活性中的作用.
了解LytD对抑制病原体,刺激植物防御和细菌殖民的贡献.

主要方法:

对 Bacillus subtilis 的基因操纵,以创建一个 lytD 破坏突变.
评估野生型和突变菌株对葡萄叶上的Plasmopara viticola生物控制的有效性.
评估对植物防御刺激和细菌生物膜形成和殖民的影响.

主要成果:

细菌细菌中lytD基因的破坏显著降低了其抑制Plasmopara viticola的能力.
这种lytD突变体对葡萄植物防御反应的刺激减弱.
发现LytD会影响生物膜的形成和葡萄叶的Bacillus subtilis殖民.

结论:

自溶素LytD在Bacillus subtilis对葡萄菌的生物控制有效性中发挥着重要作用.
LytD有助于直接抑制病原体和诱导植物免疫力.
这项研究确定了LytD作为增强Bacillus subtilis生物控制能力的潜在目标.

关键词:

自动化解素自动化解素生物膜是一种生物膜.生物控制生物控制细胞壁降解酶降解酶的细胞壁.殖民化殖民化的殖民化诱导的阻力诱导的阻力酸甘油可以是酸甘油.

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