用标准化方法生成的Yersina ruckeri盘扩散数据的流行病学切线值.
Peter Smith1, Laëtitia Le Devendec2, Eric Jouy2
1School of Natural Science, University of Galway, Galway H91 TK33 , Ireland.
Diseases of aquatic organisms
|April 25, 2024
概括
这项研究确定了在鱼类病原体Yersinia ruckeri.中进行抗菌素敏感性测试的关键解释标准. 生成的数据支持为关键抗菌剂设定协调的流行病学切线值.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 水生动物卫生 水生动物卫生
背景情况:
- 国际协调的解释标准对于抗菌素敏感性测试至关重要.
- 对于鱼类病原体Yersinia ruckeri,目前缺乏这样的标准.
- 需要标准化的标准来准确地解释Y. ruckeri的抗菌素敏感性数据.
研究的目的:
- 为了生成数据,以确定Yersinia ruckeri. 的流行病学切线值.
- 促进对Y. ruckeri. 进行国际协调的解释标准的制定.
- 支持在水产养殖中进行标准化抗微生物敏感性测试.
主要方法:
- 采用标准化的光盘扩散方法,在22°C下使用穆勒·欣顿合物,持续24-28小时.
- 在3个独立实验室中为4种抗微生物药物生成了抑制区数据.
- 在数据分析中采用基于统计的规范抵抗解释.
主要成果:
- 针对安培 (≥16毫米),佛洛芬尼科尔 (≥23毫米),氧化环素 (≥24毫米) 和三甲胺-硫甲醇 (≥30毫米) 的计算流行病学截止值.
- 已证明数据的数量和质量足以确定共识的流行病学切线值.
- 提供了对Y. ruckeri. 的协调抗微生物敏感性测试的基础.
结论:
- 生成的数据适用于建立国际协调的,共识的Y.ruckeri.流行病学切线值.
- 这项工作解决了鱼类病原体抗微生物耐药性监测中的一个关键缺口.
- 标准化标准将改善对鱼类Yersinia ruckeri感染的有效治疗.
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