用TSHR A和IGF-1Rα基因子单元免疫诱导的Graves轨道病的小鼠模型
1Department of Nuclear Medicine, General Hospital of Tianjin Medical University, 154 Anshan Road, Heping, Tianjin, 300052, China.
Journal of endocrinological investigation
|April 25, 2024
概括
这项研究成功地为Graves创建了一个小鼠模型.
科学领域:
- 内分泌学 在内分泌学.
- 免疫学 免疫学 免疫学
- 遗传学 遗传学 是一个
背景情况:
- 格雷夫斯病 (GD) 和格雷夫斯轨道病 (GO) 是一种自身免疫性疾病.
- 开发精确的动物模型对于了解疾病机制和测试疗法至关重要.
研究的目的:
- 建立一个鼠标模型的格雷夫斯病与格雷夫斯轨道病 (GD+GO) 正确反映临床特征.
- 为了评估共同注射特定塑在诱导这种模型中的有效性.
主要方法:
- 雌性BALB/c小鼠注射了编码胰岛素样生长因子1受体 (IGF-1R) α亚单元和/或甲状腺增生蛋白受体 (TSHR) A亚单元的等离子体.
- 使用电穿孔诱导模型,组的注射频率和等离子体类型 (TSHR,IGF-1Rα或两者) 不同.
- 评估结果包括激素水平,抗体标位,成像 (甲状腺和MRI),组织组织学和肌肉体积.
主要成果:
- 与单独的TSHR等离子体 (60%) 相比,两种等离子体 (GD+GO模型) 的同时注射导致更高的发病率 (83.3%).
- 在GD+GO模型中的小鼠显示T4,TSAb和TSBAb水平升高,TSH和体重降低.
- 观察到甲状腺吸收量增加和眼睛肌肉体积增加,以及甲状腺和后肌肉的炎症透和胀.
结论:
- 通过重复同时注射pcDNA3.1/IGF-1Rα和pcDNA3.1/TSHR-289等离子体,成功诱导了GD+GO小鼠模型.
- pcDNA3.1/IGF-1Rα单独没有诱导GD,而联合注射导致比单独的TSHR等离子体更严重的GD+GO.
相关概念视频
01:28Graves' Disease I: Introduction
Graves' disease is an autoimmune disorder that causes hyperthyroidism, or overactivity of the thyroid gland. It results from autoantibodies called thyroid-stimulating immunoglobulins (TSIs), which bind to thyroid-stimulating hormone (TSH) receptors, leading to overstimulation of hormone production and a hypermetabolic state.EtiologyAlthough considered idiopathic, Graves’ disease has well-established contributing factors. There is a strong genetic component, with increased prevalence in...
01:24Graves Disease II: Pathophysiology
Graves’ disease is an autoimmune disorder characterized by the production of thyroid-stimulating immunoglobulins (TSI) that activate TSH receptors, leading to excessive synthesis and release of thyroid hormones (T3 and T4) and resulting in hyperthyroidism.Among all causes of hyperthyroidism, Graves’ disease is the most common and can happen at any age, though it is more frequent in women. It produces a hypermetabolic state with features such as weight loss, tachycardia, tremor, and heat...