相关实验视频

Updated: Jun 27, 2025

A Mouse Model for Laser-induced Choroidal Neovascularization

A Mouse Model for Laser-induced Choroidal Neovascularization

Published on: December 27, 2015

miR-199a-5p通过调节Wnt7b/Wnt/β-catenin信号通路来调节胆道新血管化

Yu Geng¹, HaiRong Hua², Yuan Xia¹

¹Department of Ophthalmology, First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Kunming, 650032, Yunnan, China.

Journal of molecular histology

|April 25, 2024

概括

微RNA-199a-5p (miR-199a-5p) 通过向Wnt7b来抑制冠状腺新血管化 (CNV),从而抑制血管生成并促进视网膜内皮细胞的亡. 这表明miR-199a-5p是CNV的潜在治疗标.

科学领域:

眼科医生眼科眼科
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

胆道新血管化 (CNV) 是各种 fundus 疾病中视力丧失的主要原因.
微RNAs (miRNAs) 在调节CNV病变发生过程中起着至关重要的作用.
尽管其在内皮细胞血管化中的已知功能,但miR-199a-5p在CNV中的特定作用仍然在很大程度上未被阐明.

研究的目的:

研究miR-199a-5p在胆道新血管化 (CNV) 的发展中的作用和机制.
探索miR-199a-5p作为CNV治疗标的潜力.

主要方法:

通过使用高分辨率血管学建立和分析了激光诱导的CNV大鼠模型.
使用RT-qPCR和西式涂抹测量了miR-199a-5p,Wnt7b和Wnt/β-catenin通路蛋白的表达水平.
在体外测试 (CCK-8,伤口愈合,Transwell,管形成,流动细胞计) 评估了miR-199a-5p和Wnt7b对人类视网膜微血管内皮细胞 (HRMEC) 的功能影响.
使用TargetScan数据库和双露西法酶报告员测试进行了目标验证.

主要成果:

在CNV大鼠模型中,Wnt7b表达显著上调.
击败Wnt7b抑制了HRMEC的增殖,迁移,入侵和血管生成,同时促进了细胞亡.
据证实,miR-199a-5p直接针对的是Wnt7b.
过度表达miR-199a-5p通过降低Wnt7b.b的调节来抑制HRMEC血管生成和诱导亡.
在体内,Wnt7b部分挽救了miR-199a-5p抑制的抗CNV作用.
发现Wnt7b可以激活Wnt/β-catenin信号通路,促进血管生成.

结论:

miR-199a-5p通过抑制Wnt7b/Wnt/β-catenin信号通路,在人类视网膜的微血管内皮细胞中产生抗血管性和亲子亡性作用.
这些发现突出了miR-199a-5p作为治疗胆道新血管化的潜在治疗剂.

关键词:

血管新生的产生.冠状腺新血管化疗法核武器的扩散没有家庭成员7b.在 miR-199a-5p 中.

更多相关视频

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Published on: August 6, 2020

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...