儿童预制冠的生物相容性和耐酸性:一个体外研究
T Hogerheyde1, D Coates2, L Walsh1
1School of Dentistry, The University of Queensland, 288 Herston Road, Herston, 4006, Australia.
概括
评估了人类牙纤维细胞与儿科冠的生物相容性. 不钢皇冠表现出细胞毒性,并释放金属离子,与石不同,需要进一步研究层皇冠.
科学领域:
- 生物材料科学 生物材料科学
- 牙科材料 牙科材料
- 细胞生物学 细胞生物学
背景情况:
- 儿科牙科修复需要具有出色生物相容性和耐久性的材料.
- 预制冠通常用于儿科牙科,但它们对口腔组织的长期影响尚未完全理解.
- 了解特定物质的离子释放和细胞反应对于患者的安全至关重要.
研究的目的:
- 评估人类牙纤维细胞 (HGFs) 与预制儿科冠状 (PPCs) 的体外生物相容性.
- 为了评估PPC对模拟口腔条件的酸暴露的耐药性.
- 在酸性条件下研究不同PPC材料的离子释放.
主要方法:
- 评估了初级HGF的活力,代谢活性,细胞毒性和亡,这些都是在不钢,复合树脂和材料上进行的.
- 在37°C的温度下,PPC暴露于乳酸,酸和酸7天.
- 使用感应合等离子光学发射光谱学量化离子释放量.
主要成果:
- 在48小时和72小时后,在不钢和复合树脂材料上观察到HGF活力显著下降和细胞毒性增加.
- 单立体石皇冠表现出最小的离子释放.
- 面预制金属冠子释放了大量的金属离子 (Cu,Fe,Ni,Zn) 进入化物中.
结论:
- 不钢和复合树脂材料表明对HGFs的潜在细胞毒性.
- 在模拟的口服酸性条件下,由于大量的金属离子释放而引起了人们的关注.
- 由于长期暴露在酸性口腔环境中,需要进一步调查层预制金属冠的离子释放情况.
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