使用连续方形波电压测量对基于aptamer的电化学传感器进行单一电压测量扫描校准无质询
Sanduni W Abeykoon1, Ryan J White1
1Department of Chemistry, University of Cincinnati, Cincinnati, Ohio 45221-0172, United States.
Analytical chemistry
|April 25, 2024
概括
连续方形波电压测量 (cSWV) 能够实现无校准的基于aptamer (E-AB) 的电化学传感. 这种方法可以提高ATP和托布拉米辛等小分子的实时监测精度.
科学领域:
- 电化学 电化学 电化学
- 生物传感器是一种生物传感器.
- 分析化学 分析化学
背景情况:
- 基于电化学体 (E-AB) 的传感器可以实时监测生物分子.
- 传统的E-AB传感器由于基底信号而难以绝对量化.
- 现有的方法在复杂的生物矩阵中缺乏精度.
研究的目的:
- 引入连续方形波电压测量 (cSWV) 以提高E-AB传感器性能.
- 为了实现无校准传感,并提高 E-AB 传感器的时间分辨率.
- 在各种介质中证明分析物的精确度预测.
主要方法:
- 利用连续方形波电压测量 (cSWV) 进行高频电流数据收集 (100 kHz).
- 实施了双频方法,以比度电流响应来消除传感器变化.
- 在一系列度范围内测试了ATP和托布拉米辛的E-AB传感器.
主要成果:
- cSWV从单个扫描中生成多个伏特ammograms,改进了信息内容.
- 双频方法实现了无校准传感,减少了传感器对传感器的变化.
- 在缓冲和复杂介质中获得了高达±5%的误差准确的度预测.
结论:
- cSWV显著提高了E-AB传感器的精度和时间分辨率.
- 使用cSWV可以实现无校准量化,克服了传统方法的局限性.
- 这种技术为实时监测生物样本中的小分子提供了一个强大的平台.
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