基因组因素决定了Saccharomycotina酵母中的碳和代谢利基范围
Dana A Opulente1,2,3, Abigail Leavitt LaBella4,5,6, Marie-Claire Harrison4,5
1Laboratory of Genetics, Wisconsin Energy Institute, Center for Genomic Science Innovation, J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, Madison, WI 53726, USA.
概括
酵母的宽度或利用多样资源的能力主要取决于它们的内部遗传构成,而不是效率与宽度之间的权衡. 这一发现突显了内在基因组因素在微生物适应中的作用.
科学领域:
- 微生物学
- 进化生物学
- 基因组学
背景情况:
- 有机体表现出广泛的生态利基范围,从狭窄利基的专家到广泛利基的通用主义者.
- 两个主要理论解释了利基范围的变化:性能效率与范围之间的权衡,以及环境和基因组因素的综合影响.
研究的目的:
- 调查真菌分类Saccharomycotina的利基宽度变化的进化驱动因素.
- 为了确定是否权衡或内在基因组因素在塑造酵母的宽度方面具有更大的影响.
主要方法:
- 来自Saccharomycotina酵母的1051个物种 (1154个菌株) 的基因组,代谢和生态数据.
- 在24个不同的环境条件下培养酵母菌株以评估碳利用能力.
- 分析了基因构成,代谢途径和观察到的利基范围之间的关系.
主要成果:
- 在酵母物种中观察到碳利用特征 (利基范围) 的显著差异.
- 利基范围的差异与编码特定代谢途径的内在基因变异密切相关.
- 只有有限的证据支持性能效率和利基范围之间的权衡假设.
结论:
- 内在基因组因素是酵母中利基范围变化的主要决定因素.
- 这项研究提供了强有力的证据,表明内部遗传差异,而不是外部的权衡,推动了微生物的生态专业化或泛化.
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