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MdMYB44-MdTPR1抑制复合物通过基因素脱乙烯化抑制MdCCD4和MdCYP97A3的表达,以调节果中的胡卜素生物合成

Benchang Huang^1,2, Yuchen Li^1,2, Kun Jia^1,2

¹College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, China.

The Plant journal : for cell and molecular biology

|April 25, 2024

概括

果类胡卜素的生物合成是由MdMYB44和MdTPR1通过基因素脱乙来调节的. 这种机制通过调节关键的胡卜素基因来影响植物颜色,揭示了含有EAR的转录因子的普遍作用.

科学领域:

植物分子生物学植物分子生物学
生物化学生物化学
遗传学是一个遗传学.

背景情况:

胡卜素是重要的植物颜料和抗氧化剂.
在果中通过含有EAR的转录因子 (TF) 在胡卜素生物合成中,TOPLESS (TPL/TPR) 介导的基因组脱甲基化的作用尚不清楚.
MdMYB44是一个具有EAR压制图案的TF.

研究的目的:

为了阐明果胡卜素生物合成中MdMYB44-MdTPR1-介导的基因素脱甲基化的分子机制.
为了确定参与这种调节途径的基因.

主要方法:

在果 (Malus domestica) 中进行功能分析.
分子测试用于研究基因表达和蛋白质相互作用.
基因素脱乙化试验. 基因素脱乙化试验.

主要成果:

确定了MdCCD4和MdCYP97A3作为MdMYB44介导的胡卜素生物合成中的关键基因.
MdMYB44抑制了MdCCD4,增强了β分支类胡卜素生物合成.
MdMYB44抑制了MdCYP97A3,从而抑制了黄蛋白水平.
MdMYB44通过其EAR动机与MdTPR1相互作用,通过基因脱甲基化抑制基因表达.

结论:

MdTPR1-MdMYB44抑制级联调节果中的胡卜素生物合成.
这项研究提供了关于基因组脱乙化在胡卜素生产中的作用的见解.
含有EAR的TF/TPL复合物在植物物种中通过基因脱甲基化抑制基因表达具有普遍作用.

关键词:

CCD4 CCD4 这是一个很好的方法.在CYP97A3A3中.在 EAR 图案中,使用 EAR 图案.转录因子MYB转录因子顶部没有顶部胡卜素生物合成胡卜素生物合成基因素脱乙化基因素脱乙化

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