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在上皮层-半细胞过渡过程中单细胞蛋白共变的动力学
Saad Khan1,2, Rachel Conover1, Anand R Asthagiri1,2,3
1Department of Bioengineering, Northeastern University, Boston, Massachusetts 02115, United States.
Journal of proteome research
|April 25, 2024
概括
单个细胞中的蛋白质相关动态揭示了生理过渡期间的生物调节,例如上皮层-介质细胞过渡 (EMT). 这种方法揭示了参与细胞过程的蛋白质模块,提供了超出平均蛋白质水平的见解.
科学领域:
- 细胞生物学 细胞生物学
- 系统生物学 系统生物学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 身体过程,包括表皮-介质细胞过渡 (EMT),涉及复杂的蛋白质相互作用网络.
- 蛋白质相互作用的变化可能比平均蛋白质丰度更有信息,以了解细胞动态.
- 单细胞分析提供了一个强大的镜头来观察生物系统中的异质性.
研究的目的:
- 为了调查细胞群中的蛋白质共变是否比平均蛋白质丰度更好地反映了生理过渡期间的动态变化.
- 探索单细胞中蛋白质相关性动态的实用性,以识别功能性蛋白质模块.
主要方法:
- 在接受EMT的人类单个细胞中的蛋白质的量化.
- 对单细胞蛋白质数据的协变分析的应用.
- 在功能连贯的集群中对蛋白质-蛋白质相关性的时间分析.
主要成果:
- 在EMT期间,功能连贯的蛋白质集群在蛋白质-蛋白质相关性中表现出动态变化.
- 这些相关动态发生在集群的平均蛋白质丰度没有显著变化的情况下.
- 随着时间的推移,观察到的蛋白质相关动态是单调的,并确定了参与actin细胞骨架组织,能量代谢和蛋白质运输的特定蛋白质模块.
结论:
- 单个细胞中的蛋白质相关动态在生理转变期间提供了敏感的生物调节指标.
- 这种方法揭示了功能性蛋白质模块和调节机制,这些功能性蛋白质模块和调节机制是通过分析平均蛋白质丰度来掩盖的.
- 单细胞蛋白共变分析为动态细胞过程提供了宝贵的窗口.
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