在裂变酵母中,Bqt4影响SPB和核细胞之间的相对运动
Kaiyu Wang1, Hiroaki Ito1, Junko Kanoh2
1Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
Biochemical and biophysical research communications
|April 25, 2024
概括
内核膜蛋白Bqt4对于核运动至关重要. 它的缺失破坏了螺旋杆体 (SPB) 和核的相对位置,影响了核动力学.
科学领域:
- 细胞生物学 细胞生物学
- 核动力学 核动力学
- 分子遗传学 分子遗传学
背景情况:
- 核运动对于各种细胞过程至关重要,包括DNA修复和癌症预防.
- 在Schizosaccharomyces pombe中,内部核膜蛋白Bqt4调解了端粒与核外的连接.
- 核部件运动的变化反映了整体核动态的变化.
研究的目的:
- 研究内部核膜蛋白Bqt4在核运动动态中的作用.
- 确定Bqt4在维持杆体 (SPB) 与核细胞之间的相对运动中所涉及的特定领域和功能.
主要方法:
- 在Schizosaccharomyces pombe中删除bqt4+基因的突变性.
- 测量核中心和SPB (MSD ((SPB-Nucleolus)) 之间的平均平方位移 (MSD).
- 对具有改变Rap1结合和DNA结合能力的Bqt4突变的分析,包括双重突变和截断版本.
主要成果:
- 删除bqt4+显著减少了MSD (SPB-Nucleolus),表明核运动发生了变化.
- 观察到的MSD降低 (SPB-Nucleolus) 取决于微管.
- 影响Bqt4Rap1结合或非特异性DNA结合能力的突变物没有显示MSD (SPB-Nucleolus) 的显著下降.
- 与完全删除相比,一个缺少Rap1结合和DNA结合能力的截断Bqt4突变体也显示了MSD (SPB-Nucleolus) 的减少.
结论:
- Bqt4的C端域在维持SPB与核细胞之间的特征相对运动方面具有至关重要的,但未知的功能.
- Bqt4在核运动中的作用与其与微管,Rap1和非特异性DNA结合的相互作用有关,但其C端功能至关重要.
- 这些发现揭示了控制核定位和动态的复杂机制.
相关概念视频
Forces Acting on Chromosomes
3.3K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
3.3K
Destabilization of Microtubules
2.7K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.7K
Spindle Assembly
3.6K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
3.6K
The Spindle Assembly Checkpoint
3.1K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.1K
Microtubule Formation
5.7K
Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation...
5.7K


