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来自Morinda Officinalis的细胞外囊状颗粒:通过调节MAPK信号通路而产生抗骨质疏松作用

Yue Cao¹, Xuejun Tan², Jiawen Shen²

¹The Third Clinical Medical College, Guangzhou University of Chinese Medicine, NO.261 and 263, Longxi Avenue, Liwan District, Guangzhou, Guangdong, 510375, People's Republic of China; Department of Medical Technology, Medical College of Shaoguan University, NO. 288, University Road, Zhenjiang District, Shaoguan, Guangdong, 512005, People's Republic of China.

Phytomedicine : international journal of phytotherapy and phytopharmacology

|April 25, 2024

概括

来自Morinda Officinalis的细胞外囊状颗粒 (MOEVLPs) 显示出作为绝经后骨质疏松症 (PMOP) 的天然治疗方法的潜力. 这些颗粒通过MAPK途径促进骨质细胞增殖,为骨健康提供了一种新的治疗方法.

科学领域:

生物技术是生物技术.
药理学药理学是指药理学的学科.
细胞生物学细胞生物学

背景情况:

绝经后骨质疏松症 (PMOP) 是一种低骨质和微观结构损伤的疾病.
莫林达官 (MO) 含有具有抗PMOP活性的化合物.
来自Morinda Officinalis的细胞外囊状颗粒 (MOEVLPs) 是MO的新型活性成分,具有尚未研究的抗骨质疏松作用.

研究的目的:

调查MOEVLP对PMOP的缓解作用.
阐明MOEVLP在治疗PMOP时的潜在机制.

主要方法:

从MO中分离出MOEVLP,使用微分离心和超离心.
描述涉及TEM,流量纳米分析仪,SDS-PAGE,阿加罗斯凝电泳和薄层染色学.
在体内 (PMOP小鼠模型) 和体外 (MC3T3-E1细胞) 实验中,通过H&E,免疫组织化学,CCK-8,阿利沙林红色染色,蛋白质组学,生物信息学和西部斑点来评估疗效,安全性和机制.

主要成果:

MOEVLP成功地被隔离和表征,展现出大腿骨准和安全性.
在体内,MOEVLPs表现出抗PMOP的特性,在骨强化方面超过了阿伦德罗纳特.
在体外,MOEVLP通过MAPK途径促进骨质细胞增殖 (MC3T3-E1细胞),涉及CREB和RSK1,而不是骨质分化.

结论:

高质量的MOEVLP被分离出来,并被证明可以减轻PMOP.
通过MAPK通路,MOEVLP促进骨质细胞增殖,这表明了治疗PMOP的新机制.
作为绝经后骨质疏松症的天然治疗剂,MOEVLP具有前途.

关键词:

细胞外的类似囊泡的粒子.在 MAPK 信号通道中.在MCT3T3-E1中,我们可以使用MCT3T3-E1.莫琳达官员 (Morinda Officinalis) 是一种正式的葡萄.绝经后的骨质疏松症绝经后的骨质疏松症

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